Citrus CAPS Marker Database

Locus name: Tf0221

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 1200
Comment on PCR:
F-primer: AGTAGAACTCGCCTGAT
R-primer: TTGAAGGAGTATTTGGCTTAT

EST info

EST Accession number 1: CF418134
EST Accession number 2:

sequence-tagged site (STS seq)

STS:

ATAAGCAGGCCCTCGGCGTAAGTTGTTTACCAAGGAGGAAAAGGTTCTGCTGAATAAAAA
ACTACCTCATTTGGCAACTGCTACCTCTGTAAGTTCGTGACTGGTGTTAGTCTTGTTATT
TTGTATCTAAATTTCCTTTTTTATTTATAACTCTTAAAATGATTCAATACCAAGAAGTGG
TTTCTGCAGTTTAATCTTAGGTCAGATTGAGTAGTTAGTTAGAGCTGGAATGTTTTATAA
CAGGTATTAGTGAAAGATGGTTTTAACTTCTCAGTGAACTTGTTTGAGATTTCATACTTT
GGTGCTCTCTTTGTGTATTTGAGAATCTATATTCCTTGTTGAATTGTATTTTTCATTAAT
TAGATCCTCCATCATATTCATGGGTCAGTGGGCTT

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10025996m
Scaffold: scaffold_7
Start: 490750
End: 492882

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00488:mRNA_7.1
Scaffold: C_unshiu_00488
Start: 44813
End: 48259
Gene function: Ankyrin repeat domain-containing protein, chloroplastic

AGI map info (Shimada et al. 2014)

AGI LG: AGI-02
AGI cM: 81.212

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI057
DNA sequence around SNP1:

TTTTTTATTTATAACTCTTAAAATGATTCAATACCAA[T/G]AAGTGGTTTCTGCAGTTT
AATCTTAGGTCAGATTGAGTAGTTAGTTAGAGCTGGAATGTT

SNP2: SI225
DNA sequence around SNP2:

CTTTGTGTATTTGAGAATCTATATTCCTTGTTGAATTGTATTTTTCATTAATTAGATCCT
[C/G]CATCATATTCATGGGTCAGTGGGCTT

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: