Citrus CAPS Marker Database

Locus name: Tf0061

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 400
Comment on PCR:
F-primer: AAAGGCCTCATGCTAACAAT
R-primer: CTCAAGGGCAGAAGATAGTTC

EST info

EST Accession number 1: BQ625130
EST Accession number 2:

sequence-tagged site (STS seq)

STS:

AAAGGCCTCATGCTAACAATGAGACCATAACAAACCATAATGGTTCTGCAAATAACAGTA
GCAGTGCACCTGCTGCCAAGTAAGTCCAAAGTTGGTTTTGCGTTAAATCTTGGATTCAGT
AAGATAGAAAAATTACAATCTTCCTTATTCGGTGACAGGGCACAGGTTGTGGGTTGGCCA
CCAATTAGATCATTTAGGAAGAATTCATTGGCCACTTCGTCTAAGAATAATGATGAAGTA
GATGGAAAAGCAGGTTCCAGTGCTCTGTTTGTTAAGGTCAGCATGGATGGTGCTCCTTAT
TTAAGAAAAGTGGACTTGAAAAATTACTCTAAATATCAGGAACTATCTTCTGCCCTTGAG

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10001592m
Scaffold: scaffold_5
Start: 35159690
End: 35164384

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00764:mRNA_4.1
Scaffold: C_unshiu_00764
Start: 30899
End: 36628
Gene function: Auxin-responsive protein IAA8

AGI map info (Shimada et al. 2014)

AGI LG: AGI-09
AGI cM: 61.651

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI150
DNA sequence around SNP1:

TTGGCCACCAATTAGATCATTTAGGAAGAATTCATTGGCCACTTCGTCTAAGAATAATGA
[T/C]GAAGTAGATGGAAAAGCAGGTTCCAGTGCTCTGTTTGTTAAGGTCAGCATGGATG
GTGCT

SNP2: SI186
DNA sequence around SNP2:

AATAACAGTAGCAGTGCACCTGCTGCCAAGTAAGTCCAAAGTTGGTTTTGCGTTAAATCT
[T/C]GGATTCAGTAAGATAGAAAAATTACAATCTTCCTTATTCGGTGACAGGGCACAGG
TTGTG

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: