Citrus CAPS Marker Database

Locus name: Af1227

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 500
Comment on PCR:
F-primer: GCCCAAGCCACCTCCGATT
R-primer: GCATAAACATGATGGCGAAGT

EST info

EST Accession number 1: CV719267
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
CAACTTAATCTGCTGGAAATTCCATATGTGTTGCTTTGGCTTCAGATAGATAATTGATTA
GTAAACATAGCTATGTGATAATAATAGCTTATATGTCATGATGCTAAAACTTACTTAATA
GAGATGACATGTGATCACCTTGAGAGGTTCGTTTAAATAGAGTTAATTTGAAAGAGATTT
TGTAGAAACACGTTGAAATAGAAGCATCTGTGGTTCTTGCTTAGTTCTTTTATTTACTTC
CATCAAATACTGTAAAACCGAGGATTAATTGTCTTTAGCTCTGGGAATTTACTTTTTACA
GTGTAAAGAATCTGAGAATCAGATATAGTCCACCAGAAAGCTACAATCATATAGTAGTGA
TCATCTAGTAGAAATAGACAAACACAAGGCACGTTAAAACTCTTAAACCTCAGGATGCAA
CATGGTTTGTTTGAATTTTAACTTGATCCAAGGTAAAAATAAGGTGTGAAAGGTTGCTCC
TTTTCTTACAAAAAGGTTTGAAAGGTTGCAATTTGAGTTAATATTTGGACATAGACAGGA
GTGTTT
STS source: Clementine haploid
STS Forward / Reverse: Foward read
STS:
AAATCAAAATTACATAACTCATTTAACTTCTTCGATCTCATCCAATGCATAGTTGTTCGT
CGATACATTTGCATAGTTAACCTTGTTGAATCGAACCACAACTGGGTACCGACTCTTGGG
ATCCTACAAGATTTTAAACAGAATGTTTTAGAAACTCATTTAAAATGATTGAACGTTACA
TAAACGAGAAAAATAGAGAACAATCTGACCTGGTCAACAGCAACCACAGATCCAATGCCA
TTGTACCAGTAGGACTCCCTCCTGAGAATCTTAACCTGATGAGTCAAAAGCAGAAAATCT
TGATTAGAATGGTGGATCAAGAAACACTCCTGTCTATGTCCAAATATTAACTCAAATTGC
AACCTTTCAAACCTTTTTGTAAGAAAAGGAGCAACCTTTCA
STS source: Clementine haploid
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10022750m
Scaffold: scaffold_3
Start: 42672043
End: 42673648

Target on C. unshiu (v1.0)

AGI map info (Shimada et al. 2014)

KmMg map info (Omura et al. 2003)

JtTr map info (Omura et al. 2003)

Km/O41 map info (Nakano et al. 2003)

Rp/Sa map info (Ohta et al. 2011)

CAPS genotyping (Hybrid cv/Citrus collection)

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)